正常人結腸上皮細胞;FHC STR
- 別名:
- 編碼:FBQ1053A
- 級別:
- 簡介:培養(yǎng)條件:he base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006).To make the complete growth medium, add the following components to the base
基礎信息
中文名稱/STR | 正常人結腸上皮細胞;FHC STR |
產品介紹
培養(yǎng)條件:he base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006).To make the complete growth medium, add the following components to the base medium:
extra 10 mM HEPES (for a final conc. of 25 mM)
10 ng/ml cholera toxin
0.005 mg/ml insulin
0.005 mg/ml transferrin
100 ng/ml hydrocortisone
20 ng/mL human recombinant EGF (Thermo Fisher PHG0311)
Fetal Bovine Serum, 10% final conc (ATCC 30-2020)
傳代方法:1:3傳代,2-3天換液一次
凍存條件:無血清細胞凍存液
STR:Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12,13 D16S539: 9,11 D5S818: 12,13 D7S820: 8,12 THO1: 6,9.3 TPOX: 8,11 vWA: 16
extra 10 mM HEPES (for a final conc. of 25 mM)
10 ng/ml cholera toxin
0.005 mg/ml insulin
0.005 mg/ml transferrin
100 ng/ml hydrocortisone
20 ng/mL human recombinant EGF (Thermo Fisher PHG0311)
Fetal Bovine Serum, 10% final conc (ATCC 30-2020)
傳代方法:1:3傳代,2-3天換液一次
凍存條件:無血清細胞凍存液
STR:Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12,13 D16S539: 9,11 D5S818: 12,13 D7S820: 8,12 THO1: 6,9.3 TPOX: 8,11 vWA: 16
質量指標
鑒定 | STR鑒定 |
形態(tài)特性 | 上皮樣 |
生長特性 | 貼壁生長 |
MSDS
相關資料
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